Glossary entry (derived from question below)
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Aug 20, 2023 11:45
9 mos ago
18 viewers *
English term
Flasher
English to Spanish
Science
Medical: Instruments
Instrumentos de laboratorio
Es un artículo sobre un ensayo de laboratorio sobre parvovirus y virus de la hepatitis A, y esta es la sección en donde se definen ciertos términos.
La oración es la siguiente:
Calibrators: Negative and Positive Calibrators used to determine run validity as well as the *flasher* (Internal Control and HAV) cutoffs and the glower (Analyte) calibration curve.
No termino de determinar si es un tipo de controlador. Se me hace que "flasher cutoffs" serían los cortes o umbrales del controlador, pero es más una conjetura que otra cosa...
Desde ya, ¡mil gracias!
La oración es la siguiente:
Calibrators: Negative and Positive Calibrators used to determine run validity as well as the *flasher* (Internal Control and HAV) cutoffs and the glower (Analyte) calibration curve.
No termino de determinar si es un tipo de controlador. Se me hace que "flasher cutoffs" serían los cortes o umbrales del controlador, pero es más una conjetura que otra cosa...
Desde ya, ¡mil gracias!
Proposed translations
(Spanish)
| 4 -1 | intermitentes |
Arturo Oswal Alva Mendiburu
|
References
| multiple sources Nernst Lamp |
Tomasso
|
Change log
Aug 22, 2023 08:05: dcanossa Created KOG entry
Proposed translations
-1
6 hrs
Arturo Oswal Alva Mendiburu
Peru
Peru
Native in Spanish
Peru
Spanish translator and subtitler
Native in: Spanish (Variants: Latin American, Peruvian) 
Works in: English to Spanish, Spanish to English, Spanish
intermitentes
Se refiere los intermitentes que poseen los calibradores del tipo positivos y negativos que se usan para determinar la validez de las pruebas cuando se usan curvas de calibración con señales luminosas en el analito y cortes de control interno en los intermitentes.
Peer comment(s):
| disagree |
abe(L)solano
: habiendo trabajado 20 años en laboratorio nunca oí este término. "flasher" es en sentido figurado, se refiere a los límites establecidos.
13 hrs
|
Reference comments
4 hrs
Reference:
multiple sources Nernst Lamp
Lampara de Nernst
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Note added at 1 day 5 hrs (2023-08-21 17:37:25 GMT)
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Not my field, encontre explicacion de ANALYTE,
Abstract
Calibration curve is a regression model used to predict the unknown concentrations of analytes of interest based on the response of the instrument to the known standards. Some statistical analyses are required to choose the best model fitting to the experimental data and also evaluate the linearity and homoscedasticity of the calibration curve. Using an internal standard corrects for the loss of analyte during sample preparation and analysis provided that it is selected appropriately. https://www.intechopen.com/chapters/58596
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Note added at 1 day 6 hrs (2023-08-21 17:46:49 GMT)
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Es cierto, no tiene que ver con lampa Nernst, parece que estan usando forma corta de Glower signal, y Flasher signal...
encontre esto si le sirve...Internal Control is added to each test specimen and assay calibrator tube via the working Target Capture Reagent (wTCR) that contains the Internal
Control. The Internal Control in this reagent controls for specimen processing, amplification, and detection steps. Internal Control signal in each tube or
assay reaction is discriminated from the HIV/HCV/HBV signal by the differential kinetics of light emission from probes with different labels.37 Internal
Control-specific amplicon is detected using a probe with rapid emission of light (termed a **“flasher signal”)***. Amplicon specific to HIV/HCV/HBV is
detected using probes with relatively slower kinetics of light emission (termed a “glower signal”). The Dual Kinetic Assay (DKA) is a method used to
differentiate between the signals from** flasher and glower labels***.37 When used for the simultaneous detection of HIV, HCV, and HBV, the Procleix Ultrio
Elite Assay differentiates between Internal Control and combined HIV/HCV/HBV signals but does not discriminate between individual HIV, HCV, and
HBV signals. https://www.fda.gov/media/112861/download
--------------------------------------------------
Note added at 1 day 5 hrs (2023-08-21 17:37:25 GMT)
--------------------------------------------------
Not my field, encontre explicacion de ANALYTE,
Abstract
Calibration curve is a regression model used to predict the unknown concentrations of analytes of interest based on the response of the instrument to the known standards. Some statistical analyses are required to choose the best model fitting to the experimental data and also evaluate the linearity and homoscedasticity of the calibration curve. Using an internal standard corrects for the loss of analyte during sample preparation and analysis provided that it is selected appropriately. https://www.intechopen.com/chapters/58596
--------------------------------------------------
Note added at 1 day 6 hrs (2023-08-21 17:46:49 GMT)
--------------------------------------------------
Es cierto, no tiene que ver con lampa Nernst, parece que estan usando forma corta de Glower signal, y Flasher signal...
encontre esto si le sirve...Internal Control is added to each test specimen and assay calibrator tube via the working Target Capture Reagent (wTCR) that contains the Internal
Control. The Internal Control in this reagent controls for specimen processing, amplification, and detection steps. Internal Control signal in each tube or
assay reaction is discriminated from the HIV/HCV/HBV signal by the differential kinetics of light emission from probes with different labels.37 Internal
Control-specific amplicon is detected using a probe with rapid emission of light (termed a **“flasher signal”)***. Amplicon specific to HIV/HCV/HBV is
detected using probes with relatively slower kinetics of light emission (termed a “glower signal”). The Dual Kinetic Assay (DKA) is a method used to
differentiate between the signals from** flasher and glower labels***.37 When used for the simultaneous detection of HIV, HCV, and HBV, the Procleix Ultrio
Elite Assay differentiates between Internal Control and combined HIV/HCV/HBV signals but does not discriminate between individual HIV, HCV, and
HBV signals. https://www.fda.gov/media/112861/download
Discussion
"El amplicón específico del HPV se detecta usando sondas
con una cinética de emisión de luz relativamente más lenta (glower). El ensayo de cinética doble (Dual Kinetic Assay, DKA) es un método utilizado para diferenciar entre las señales <B>procedentes de los marcadores flasher y glower.44"
Al parecer es terminología específica de este sistema.
NO USAN LÁMPARA DE NERSNT EN ESTE PROTOCOLO.
replicates is valid, the Procleix Panther System Software will automatically invalidate the run.
4. In a valid run,** cutoff values** will be automatically calculated for Internal Control (flasher**) and analyte (**glower). pagina 15
tmb pagina 23 All calculations described above are performed by the Procleix Panther System Software. Two cutoffs are determined for each assay: one for the
Analyte Signal (***glower signal***) termed the Analyte Cutoff and one for the Internal Control Signal (***flasher signal) termed the Internal Control Cutoff. The
calculation of these cutoffs is shown above. For each sample, an Analyte Signal RLU value and Internal Control Signal RLU value are determined.
Analyte Signal RLU divided by the Analyte Cutoff is abbreviated as the Analyte Signal/Cutoff (S/CO) on the report.
Hay miles de metodologías en uso, no dices cuál exactamente están usando. También es conjetura lo de la lámpara de Nernst...