Glossary entry (derived from question below)
English term or phrase:
O-solution
English answer:
Solution containing oxygen
Added to glossary by
Ariana Ariana
Nov 13, 2021 08:48
2 yrs ago
18 viewers *
English term
Uzbekistan
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Native in: Russian (Variant: Standard-Russia) 
Works in: English to Russian, French to Russian, Italian to Russian, and 4 more.
O-solution
English
Medical
Medical (general)
ARTICLE
Equal amounts (40 mg) of proteins were denatured in O-solution, boiled for 10 minutes, and then
separated on 10% sodium dodecyl sulfate–polyacrylamide gel. Proteins were then transferred on a nitrocellulose membrane and detected by primary antibodies, respectively, rabbit polyclonal antihuman/mouse b2-adrenergic receptor (1:100, predicted band size 46 kDa; Abcam) and mouse monoclonal antihuman b-actin (1:1000; Abcam).
separated on 10% sodium dodecyl sulfate–polyacrylamide gel. Proteins were then transferred on a nitrocellulose membrane and detected by primary antibodies, respectively, rabbit polyclonal antihuman/mouse b2-adrenergic receptor (1:100, predicted band size 46 kDa; Abcam) and mouse monoclonal antihuman b-actin (1:1000; Abcam).
Responses
1 hr
Selected
Solution containing oxygen
During redox signaling, H2O2 oxidizes the thiolate anion to sulfenic form (Cys-SOH) causing allosteric changes within the protein that alter its function https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055301/
When a solution of a protein is boiled, the protein frequently becomes insoluble—i.e., it is denatured—and remains insoluble even when the solution is cooled. - https://www.britannica.com/science/protein/Protein-denaturat...
Protein solutions usually contain buffer and salt as well as protein and water molecules. The important point is that the third component causes the increase in the solution viscosity by the exclusion volume effect. - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182935/
When a solution of a protein is boiled, the protein frequently becomes insoluble—i.e., it is denatured—and remains insoluble even when the solution is cooled. - https://www.britannica.com/science/protein/Protein-denaturat...
Protein solutions usually contain buffer and salt as well as protein and water molecules. The important point is that the third component causes the increase in the solution viscosity by the exclusion volume effect. - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182935/
Note from asker:
| Thank you |
Peer comment(s):
| neutral |
philgoddard
: I don't see the relevance of any of your references.
3 hrs
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Deductions, comparing: all relationated with solution containing oxigene: think alittle and yo'll, not at the first sight
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| neutral |
liz askew
: neither do I. and I cannot find this in relation to the asker's context
4 hrs
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Reactive oxygen species (ROS) are byproducts of aerobic metabolism. ROS include the superoxide anion (O2−), hydrogen peroxide (H2O2), and: think a little and you'll arrive to solutioon containing oxigen, there is no direct information -https://www.ncbi.n
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4 KudoZ points awarded for this answer.
Comment: "Thank you"
-1
12 hrs
organic compounds solution / a brand name
Protein Denaturation Methods:
Use of organic compounds, such as ethyl alcohol:
These compounds are capable of engaging in intermolecular hydrogen bonding with protein molecules, disrupting intramolecular hydrogen bonding within the protein.
https://chem.libretexts.org/Courses/University_of_Arkansas_L...
Since the "O" in O-solution is written in capital letter. It could also be the brand name of a kind of solution.
Use of organic compounds, such as ethyl alcohol:
These compounds are capable of engaging in intermolecular hydrogen bonding with protein molecules, disrupting intramolecular hydrogen bonding within the protein.
https://chem.libretexts.org/Courses/University_of_Arkansas_L...
Since the "O" in O-solution is written in capital letter. It could also be the brand name of a kind of solution.
Peer comment(s):
| neutral |
philgoddard
: I agree with Yvonne.
8 hrs
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| disagree |
Yvonne Gallagher
: it is pure guesswork and you have 2 separate "answers" here so which do you think it is?
14 hrs
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The asker has more context. He / She will determine which one is the best.
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Discussion
Proteins are denatured by treatment with alkaline or acid, oxidizing or reducing agents, and certain organic solvents. Interesting among denaturing agents are those that affect the secondary and tertiary structure without affecting the primary structure.