Bacterias: siembra de 10 ml de la dilución 1/10

English translation: Bacteria: inoculation with 10 mL from the 1:10 dilution

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
Spanish term or phrase:Bacterias: siembra de 10 ml de la dilución 1/10
English translation:Bacteria: inoculation with 10 mL from the 1:10 dilution

22:27 Mar 9, 2016
    The asker opted for community grading. The question was closed on 2016-03-13 08:54:08 based on peer agreement (or, if there were too few peer comments, asker preference.)


Spanish to English translations [PRO]
Medical - Biology (-tech,-chem,micro-) / microbiología
Spanish term or phrase: Bacterias: siembra de 10 ml de la dilución 1/10
yo he traducido al inglés:

Bacteria: Inoculating of 10 mL of the 1/10 dilution

Ruego me expliquéis si lo he hecho bien. He mirado en Google y también en el IATE . Pero no soy bióogo y tengo duda. Para ayudar pongo mas contexto: * Bacterias: siembra de 10 ml de la dilución 1/10 por filtración en 2 placas de agar TSA seguido de incubación a 30-35ºC durante 5 días.
* Hongos: siembra de 10 ml de la dilución 1/10 por filtración en 2 placas de agar TSA seguido de incubación a 30-35ºC durante 5 días.
Luis de Larrauri
Spain
Local time: 17:21
Bacteria: inocuation of 10 mL of/from the 1:10 dilution
Explanation:
IMO, you were correct to begin with.

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Note added at 4 hrs (2016-03-10 03:06:26 GMT)
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Here is an excerpt about the spread plate technique:

http://www.microbelibrary.org/component/resource/laboratory-...
One method of distributing bacteria evenly over the surface of an agar plate medium is commonly referred to as the spread plate method. Classically a small volume of a bacterial suspension is spread evenly over the agar surface using a sterile bent glass rod as the spreading device. The goal in evenly distributing the bacterial suspension is typically to permit the growth of colonies that can subsequently be enumerated (see Serial Dilution Protocols) and/or sampled following incubation. Each plate is spread with a single inoculum of the bacterial suspension.

An alternative approach to spreading a single inoculum volume with a smooth device is to apply a smaller volume and tip the plate, allowing gravity to distribute the inoculum in a band or track (track method) or to allow the inoculum to dry in place (drop method). With this alternative approach, several sample dilutions can be distributed on a single agar plate.
Selected response from:

Muriel Vasconcellos
United States
Local time: 09:21
Grading comment
Thank you. for helping me in this term. I appreciate your help very much LuisLarra
4 KudoZ points were awarded for this answer



Summary of answers provided
4Bacteria: inocuation of 10 mL of/from the 1:10 dilution
Muriel Vasconcellos
3Bacteria: spread plate 10 ml of the 1:10 dilution
liz askew
3Bacteria: inoculation from 10 ml of the 1:10 dilution
Robert Carter


Discussion entries: 4





  

Answers


18 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5
Bacteria: spread plate 10 ml of the 1:10 dilution


Explanation:
Volume to Volume - Wellesley College
academics.wellesley.edu/Biology/Concepts/Html/volumetovolume.html
25 Aug 2004 - For example, to make a 1:10 dilution of a 1M NaCl solution, you would ... For example: if you needed 10 mL of the 1:10 dilution, then you would ...

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Note added at 21 mins (2016-03-09 22:48:20 GMT)
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Genetic Transformation Systems in Fungi
https://books.google.co.uk/books?isbn=3319105035
Marco A. van den Berg, ‎Karunakaran Maruthachalam - 2014 - ‎Science
a10 μg of DNA in X μL (plus DNA) or X μL of water (minus DNA; as a control) ... glass tubes (about 15 mL in each) and dilute with equal volume of 1×STC. ... (d) 100 μL+900 μL of 1 ×STC; spread 100 μL of this 1:10 diluted sample (S1) on ...

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Note added at 21 mins (2016-03-09 22:48:56 GMT)
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or just

Bacteria: spread 10 ml of the 1:10 dilution

liz askew
United Kingdom
Local time: 16:21
Native speaker of: Native in EnglishEnglish
PRO pts in category: 106
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2 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5
Bacteria: inoculation from 10 ml of the 1:10 dilution


Explanation:
I think inoculation is perfectly fine here, and I imagine it's done from 10 ml rather than with, since using the whole 10 ml would be quite a lot for two agar plates.

Bacteria may be introduced to the media (inoculated) by various means. Usually the bacteria e.g. from a drop in a heat-sterilised loop are spread on the surface of (ready set) agar.
http://www.biotopics.co.uk/microbes/tech1.html

Un microorganismo se puede sembrar en un medio líquido o en la superficie de un medio sólido de agar.
https://es.wikipedia.org/wiki/Cultivo_(microbiología)

Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the best temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius for cultures from humans or animals, or lower for environmental cultures).
https://en.wikipedia.org/wiki/Microbiological_culture


Robert Carter
Mexico
Local time: 10:21
Native speaker of: Native in EnglishEnglish

Peer comments on this answer (and responses from the answerer)
neutral  Muriel Vasconcellos: You make a good point. The text doesn't say what the dilution is. It could be dilution of the agar itself. The inoculum is usually a much smaller amount. But the syntax is telling me that 10 mL is the inoculum.
1 hr
  -> What makes you to think that Muriel? 5 ml per agar plate seems a lot to me, am I off-base here?//Okay, I don't agree about the syntax, but thanks anyway for giving me your rationale. Saludos.
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3 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
Bacteria: inocuation of 10 mL of/from the 1:10 dilution


Explanation:
IMO, you were correct to begin with.

--------------------------------------------------
Note added at 4 hrs (2016-03-10 03:06:26 GMT)
--------------------------------------------------

Here is an excerpt about the spread plate technique:

http://www.microbelibrary.org/component/resource/laboratory-...
One method of distributing bacteria evenly over the surface of an agar plate medium is commonly referred to as the spread plate method. Classically a small volume of a bacterial suspension is spread evenly over the agar surface using a sterile bent glass rod as the spreading device. The goal in evenly distributing the bacterial suspension is typically to permit the growth of colonies that can subsequently be enumerated (see Serial Dilution Protocols) and/or sampled following incubation. Each plate is spread with a single inoculum of the bacterial suspension.

An alternative approach to spreading a single inoculum volume with a smooth device is to apply a smaller volume and tip the plate, allowing gravity to distribute the inoculum in a band or track (track method) or to allow the inoculum to dry in place (drop method). With this alternative approach, several sample dilutions can be distributed on a single agar plate.

Muriel Vasconcellos
United States
Local time: 09:21
Works in field
Native speaker of: Native in EnglishEnglish
PRO pts in category: 43
Grading comment
Thank you. for helping me in this term. I appreciate your help very much LuisLarra
Login to enter a peer comment (or grade)



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